Fig. 13From: MicroRNA-221-3p is related to survival and promotes tumour progression in pancreatic cancer: a comprehensive study on functions and clinicopathological valuemiR-221-3p inhibits CDKN1B expression in PC cells. a Direct binding sequences of miR-221-3p and CDKN1B mRNA. b–e Luciferase activities of 3′UTR CDKN1B-luc constructs in PC cells after transfection with mimic, inhibitor or control. The results were assessed with t tests (unpaired). f, g CDKN1B expression after transfection with mimic, inhibitor or control determined by RT-qPCR. h–k CDKN1B expression after transfection with mimic, inhibitor or control determined by western blottingBack to article page