Fig. 7

Overexpression of miR-221-3p caused elevated proliferation in PC cells. a, b RT-qPCR assay to assess relative miR-221-3p content in BxPC-3 cells after transfection with mimic and control or inhibitor and control. c, d CCK-8 assay in BxPC-3 cells 6 h after transfection with mimic and control or inhibitor and control, with results assessed every 24 h for 96 h. e, f RT-qPCR assay to assess relative miR-221-3p content in MIA PaCa-2 cells after transfection with mimic and control or inhibitor and control. g, h CCK-8 assay in MIA PaCa-2 cells 6 h after transfection with mimic and control or inhibitor and control, with results assessed every 24 h for 96 h. All experiments were conducted with 5 replicates, and the results were assessed with t tests (unpaired). In conclusion, the results revealed that miR-221-3p can promote proliferation activity in PC cells