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Fig. 4 | Cancer Cell International

Fig. 4

From: LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis

Fig. 4

BCL2 was the target of miR-34b-5p in CRC cells. a The putative binding sites of miR-34b-5p to wild type BCL2 (BCL2-WT) and the mutant BCL2 (BCL2-MUT). b, c Luciferase activity detection of HCT116 (b) and LoVo (c) cells co-transfected with miR-34b-5p mimic/miR-NC and luciferase reporters expressing BCL2-WT or BCL2-MUT. d Endogenous protein level of BCL2 in HCT116 and LoVo cells transfected with miR-NC, miR-34b-5p inhibitor or miR-34b-5p mimic were detected by western blotting. e The expressional level of BCL2 mRNA in CRC and normal tissues were examined by qRT-PCR. f Pearson’s correlation curve revealed the positive relevance between BCL2 and LINC02418 levels. g, h IHC and WB assays were performed to detect the protein level of BCL2 in CRC and normal tissues. The error bars stand for standard deviation (SD). N, normal tissue; T, tumor tissue. Data were representatives of three independent experiments with similar results; **P < 0.01; ns, no difference, compared to miR-NC group

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