Fig. 5

ALT induces apoptosis and inhibits autophagy of ALL cells. BV173 and NALM6 cells were transfected with si-AP2M1 or si-NC, and then treated with 5 μM ALT for 24 h. a Annexin/V-PI staining and flow cytometry were performed to assess cell apoptosis of ALL cells. b JC-1 staining was used to detect the mitochondrial membrane potential of the ALL cells. c LC3 fluorescence dots labeled by GFP were observed under the fluorescence microscope. d Western blotting was used to assess the expression of proteins. ^*P < 0.05. ALL, acute lymphoblastic leukemia; ALT, alantolactone; si, small interfering; NC, negative control; AP2M1, adaptor related protein complex 2 subunit mu 1