Fig. 6

Induction of autophagy reverses the pro-apoptotic effects of ALT on BV173 and NALM6 cells. ALL cells were treated with ALT either alone or combined with rapamycin for 24 h. a Annexin/V-PI staining and flow cytometry were performed to detect cell apoptosis of ALL cells. b JC-1 staining was performed to detect the mitochondrial membrane potential of the ALL cells. c LC3 fluorescence dots labeled by GFP were observed using fluorescence microscopy. d Western blotting was used to investigate the expression of autophagy-related proteins. e Western blotting was used to investigate the expression of apoptosis-related proteins. ^*P < 0.05. ALL, acute lymphoblastic leukemia; ALT, alantolactone