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Fig. 3 | Cancer Cell International

Fig. 3

From: Down-regulation of lncRNA UCA1 enhances radiosensitivity in prostate cancer by suppressing EIF4G1 expression via sponging miR-331-3p

Fig. 3

UCA1 knockdown enhanced the radiosensitivity of PCa cells. a, b The relative UCA1 expression was detected by qRT-PCR in 22RV1 (a) and DU145 (b) cells transfected with si-UCA1#1, si-UCA1#2, si-UCA1#3, or si-RNA. c–j 2RV1 and DU145 cells were transfected with si-NC or si-UCA1#3. c, d Cell viability was detected in transfected 22RV1 (c) and DU145 (d) cells at 0, 24, 48, and 72 h after Gy irradiation. e, f Transfected 22RV1 (e) and DU145 (f) cells were subjected to 0, 2, 4, 6 and 8 Gy irradiation. After 2 weeks, the colony survival fractions were measured. g, h Cell apoptosis was determined in transfected 22RV1 and DU145 cells at 24 h after 6 Gy radiotherapy. i, j The protein expression of Cyclin D1, Bcl-2, and Bax was examined in transfected 22RV1 (i) and DU145 (j) cells after 6 Gy irradiation. *P < 0.05

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