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Fig. 3 | Cancer Cell International

Fig. 3

From: Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment

Fig. 3

Conditioned medium from OPNc overexpressed cells induced cellular levels of OPN-SIs dependent to the activation of nuclear calcium signals. Nuclear Ca2+ in HT115 (a) or HCT-8 (b) cells transfected with nuGCaMP6s-tdTomato plasmid and stimulated with OPN-CMs from 0 to 40 min with representative live fluorescence images during assays. A total of 10 to 30 individual cells were selected for quantitative analyses of the ratio between GCaMP6s and tdTomato fluorescence intensities (lower). c, d Quantitative analyses in cells induced by OPNc-CM and CM with addition of OPN-neutralizing antibody. The normalised mRNA levels of opn splicing isoforms in HT115 (e) and HCT-8 (f) cells as determined by quantitative RT-qPCR after exposure in OPN-CMs for 40 min

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Cancer Cell International

ISSN: 1475-2867

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