Fig. 4

Inhibition of CaMKII or methylase activities attenuated the anti-apoptotic effects of CMs with OPN-SIs. The cell survival from CCK8 assays in HT115 (a) and HCT-8 (b) cells preadminstered with 5 μM KN-93 or 5 μg/ml 5-AZA and subjected to 6 μg/ml 5-FU in various OPN-SI CMs (n = 6). c–f Apoptosis assays in HT115 and HCT-8 cells by flow cytometry under conditions described as in (a) and (b) with 20 μg/ml 5-FU were shown in (c–f) (n = 3).The data were represent the mean values ± SD. *p < 0.05, **p < 0.01, ***p < 0.001