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Fig. 5 | Cancer Cell International

Fig. 5

From: Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment

Fig. 5

Enhanced splicing for the production of OPN-SIs was dependent to the phosphorylation of MeCP2. The mRNA levels of OPN-SIs in HT115 (a) and HCT-8 (b) cells transfected with MeCP2 siRNAs. The effect of 5-AZA on 5-FU induced OPN splicing in HT115 (c) and HCT-8 (d) cells. Cells were treated with 5 μg/ml 5-AZA or 5 μg/ml 5-AZA and 6 μg/ml 5-FU for 24 h. The OPN-SIs mRNA levels in HT115 (e) and HCT-8 (f) cells in the present of 6 μg/ml 5-FU or 5 μM KN-93 or both for 24 h. The protein levels of MeCP2 and p-MeCP2 were determined by western blotting in HT115 (g) and HCT-8 (h) cells following treatments with 6 μg/ml 5-FU or 5 μM KN-93 or both for 24 h. *p < 0.05, **p < 0.01, ***p < 0.001

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