Fig. 6

Determinization of MeCP2 binding to opn exon 4 and exon 5 by ChIP assays. a The binding of MeCP2 to alternatively spliced exon 4 and exon 5 of opn gene in non-treated HCT-8 cells, shown in agarose gel electrophoresis (left) and qPCR (right) results. The exon 10 of HAUS8 gene was used as a positive reference of MeCP2 association, and a mixture of non-specific IgG was used as the assay control. b MeCP2 binding to opn exon 4 determined by ChIP assays from HCT-8 cells treated with 6 μg/ml 5-FU, OPNc-CM, 5 μg/ml 5-AZA or 5 μM KN-93 for 24 h. c MeCP2 binding to opn exon 5 under conditions as in (b). ***p < 0.001