Table 1 The primers and siRNAs used in this study
| Â | Target | Oligonucleotide sequence |
|---|---|---|
qPCR | OPN-t | F: GCCGAGGTGATAGTGTGGTT |
R: AACGGGGATGGCCTTGTATG | ||
OPN-a | F: GCCGAGGTGATAGTGTGGTT | |
R: AACGGGGATGGCCTTGTATG | ||
OPN-b | F: ATCTCCTAGCCCCACAGAC | |
R: AAAATCAGTGACCAGTTCATCAG | ||
OPN-c | F: TGAGGAAAAGCAGAATGCTG | |
R: GTCAATGGAGTCCTGGCTGT | ||
OPN-exon4 | F: AAGATAGCCACACTCAGGCCATTTG | |
R: CAGCCTCATCTGTGGATGGCTTAAC | ||
OPN-exon5 | F: AACAAGAGGTAAGTTCTCATTTTCA | |
R: AACTTATCCGAGGAAACCTAGTATT | ||
HAUS8 | F: ACAGGGTGCCACCTCTTTCT | |
R: TGCAGGTGCTGGACTTACTG | ||
GAPDH | F: GGAGCGAGATCCCTCCAAAAT | |
R: GGCTGTTGTCATACTTCTCATGG | ||
siRNA | Scramble | Sense: UUCUCCGAACGUGUCACGUTT |
Antisense: ACGUGACACGUUCGGAGAATT | ||
MeCP2-1 | Sense: GCUUAAGCAAAGGAAAUCUTT | |
Antisense: AGAUUUCCUUUGCUUAAGCTT | ||
MeCP2-2 | Sense: GCUUCCCGAUUAACUGAAATT | |
Antisense: UUUCAGUUAAUCGGGAAGCTT |