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Fig. 3 | Cancer Cell International

Fig. 3

From: RUNX2 interacts with BRG1 to target CD44 for promoting invasion and migration of colorectal cancer cells

Fig. 3

RUNX2 interacts with BRG1 to promote CD44 transcription. a Both the siRNAs for BRG1 could effectively inhibit the expression of BRG1. b BRG1 knockdown decreased the expression of CD44. c BRG1 knockdown inhibited the sphere-forming ability of RKO cells. d Knockdown of BRG1 offset the ability of RUNX2 to promote the expression of CD44 in HT115 cells. e The analysis of RUNX2 binding to the CD44 promoter by CHIP assay in RKO cells. Genomic DNA was immunoprecipitated with anti-RUNX2 antibody, where IgG served as a negative control. The primer set specific to the RUNX2 binding site of CD44 promoter was used in real-time PCR amplification. Data are shown as means ± SE; *P<0.05, **P<0.01; NC negative control, si short interfering, RUNX2 overexpression of RUNX2

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