Fig. 4

RUNX2 interacts with BRG1. a Confocal microscopy results showing that both RUNX2 and BRG1 were expressed in the nucleus of RKO and HT115 cells. b Co-IP experiments were performed with cell lysates of HEK293T expressing RUNX2-Myc and/or BRG1-GFP. The transfected constructs are indicated in the top panel. Anti-GFP (GFP-IP; left panel) or anti-Myc (Myc-IP; right panel) antibody was used for the immunoprecipitation assay. The antibodies used for WB are described on the left panel. c Co-IP experiments were performed on RKO cell lysates. The immunoprecipitation assay of RKO cell lysates with RUNX2, BRG1, or control (IgG) antibody. RUNX2 or BRG1 was detected by WB using the indicated antibody