Fig. 1
From: CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy
TCR and CAR T-cell cancer immunotherapy. a Schematic overview of CAR and TCR T-cell construction. Isolation and expansion of human T Lymphocytes (extracted from healthy donor blood) are followed by modification of T-cells by gene-editing tools (CRISPR, ZFN,…) and transduction of T-cells with transgenic TCR or CAR gene constructs. b TCR and four generations of CAR structure. (CM: co-stimulatory molecule, Cα: constant α-chain, Cß: constant ß-chain, Vα: variable α-chain, Vß: variable ß-chain, VL: variable light chain, VH: variable heavy chain) c Engineered TCR and CAR T-cells employment in the treatment of candidate patient. TCR identifies MHC-I presenting antigens and CAR targets tumor cell surface antigens