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Fig. 2 | Cancer Cell International

Fig. 2

From: CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis

Fig. 2

Characterization of circUBE2D2 in TNBC cells. a CircUBE2D2 is located at chromosome 5q31.2 and generated by via the circularization of exon 2–5 in UBE2D2 gene. b Divergent and convergent primers were used to amplify the circular and linear UBE2D2 with cDNA or gDNA as the template in MDA-MB-231 and BT-549 cells. c Relative expression of circUBE2D2 and UBE2D2 mRNA in MDA-MB-231 and BT-549 cells after treatment with RNase R. d Relative expression of circUBE2D2 and UBE2D2 mRNA at different time points in MDA-MB-231 and BT-549 cells after treatment with actinomycin D. e Nuclear-cytoplasmic fractionation assay was performed to examine the subcellular distribution of circUBE2D2 in MDA-MB-231 and BT-549 cells. ***P < 0.001

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