Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis

Fig. 5

CircUBE2D2 serves as a sponge for miR-512-3p in TNBC cells. a CircBank and StarBase 3.0 predicted seven miRNAs containing the potential binding sites of circUBE2D2. b Biotinylated-circUBE2D2 pull-down assay was performed in MDA-MB-231 and BT-549 cells, followed by qRT-PCR to measure the enrichment of seven candidate miRNAs. c Biotinylated-miR-512-3p and control Biotinylated-miR-NC were transfected into MDA-MB-231 and BT-549 cells with circUBE2D2 overexpression, and qRT-PCR was used to test the level of circUBE2D2 in qRT-PCR immunoprecipitates. d Schematic diagram of wild-type or mutant circUBE2D2 luciferase reporter vectors. e Effects of miR-512-3p overexpression on the luciferase activity of MDA-MB-231 and BT-549 cells transfected with wild-type or mutant circUBE2D2 luciferase reporter vector. f Anti-Ago2 RIP was carried out in MDA-MB-231 and BT-549 cells transfected with miR-512-3p or miR-NC, followed by qRT-PCR to measure the level of circUBE2D2. g and h qRT-PCR was utilized to evaluate the effects of circUBE2D2 silencing or overexpression on miR-512-3p expression. I The expression difference of miR-512-3p in breast cancer tissues and normal tissues was displayed according to the data from GSE45666. j Kaplan–Meier analysis for the correlation between miR-512-3p expression and overall survival of breast cancer patients from GSE40267 data. k qRT-PCR analysis of miR-512-3p expression in tumor tissues and corresponding para-cancerous tissues. l Pearson’s correlation coefficients were applied to assess the association of miR-512-3p and circUBE2D2 in TNBC tissues. ***P < 0.001

Back to article page