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Fig. 6 | Cancer Cell International

Fig. 6

From: CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis

Fig. 6

CircUBE2D2 sponges miR-512-3p to relieve its suppression on target gene CDCA3 in TNBC cells. a The potential targets of miR-512-3p predicted by TargetScan and miRTarBase were narrowed down to three by comparison with the top 200 up-regulated genes in breast cancer from GEPIA. b The effect of miR-512-3p on the expression of three candidate genes (KIF23, PRC1, CDCA3) was determined by qRT-PCR. c GEPIA database displayed the expression of CDCA3 mRNA in breast cancer tissues and normal tissues. d Kaplan–Meier analysis uncovered the correlation between CDCA3 expression and overall survival of breast cancer patients. e The expression level of CDCA3 mRNA in tumor tissues and proximal normal tissues from 66 TNBC patients was examined through qRT-PCR. f and g Pearson test was used to analyze the correlation between CDCA3 and miR-512-3p or circUBE2D2 in TNBC tumor tissues. h Putative complementary sites of miR-512-3p on the CDCA3-3′UTR. i The dual-luciferase activity was measured in MDA-MB-231 and BT-549 cells after co-transfected wild-type or mutant CDCA3 reporter and miR-512-3p or miR-NC. j and k Western blot assay was conducted to detect the effect of miR-512-3p or circUBE2D2 on CDCA3 protein level. **P < 0.01, ***P < 0.001

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