Fig. 4

Ibr-7 induced cell apoptosis in irradiated PANC-1 and Capan2 cells. a Representative histograms showing the apoptosis of PANC-1 and Capan2 cells in the Ctrl, Ibr-7, radiation and combination groups. Cells were treated with Ibr-7 (2 μmol/L) for 24 h, exposed to 6 Gy of radiation and stained with Annexin V-FITC and PI after another 24 h. b The percentages of apoptotic cells in each group were quantified. c Cells were pretreated with Ibr-7 for 24 h and then exposed to radiation for another 24 h. Western blot analysis was performed to detect the expression of PARP, Cleaved caspase 3, β-actin was used as a loading control. d The ratio of PARP/β-actin, Cleaved caspase 3/β-actin was quantified by densitometry based on immunoblot images. The results shown are the mean ± SE of 3 independent experiments. Significance was determined by Student’s t-test (*p < 0.05 compared with the combination group)