Fig. 3

MiR-324-3p inhibitor overturned the effects of MALAT1 silencing on HCT116/OxR and HCT8/OxR cells. a The predictive binding sites between MALAT1 and miR-324-3p. b, c Dual-luciferase reporter analysis for the luciferase activities of WT-MALAT1 and MUT-MALAT1 in Ox-resistant CRC cells. d The expression of miR-324-3p in Ox-resistant CRC tissues (n = 40) and Ox-sensitive CRC tissues (n = 40). e The relationship between MALAT1 and miR-324-3p in Ox-resistant CRC tissues (n = 40). f The level of miR-324-3p in Ox-resistant CRC cells, CRC cells and FHT cells. g The transfection efficiency of anti-miR-324-3p in Ox-resistant cells. h–p HCT116/OxR and HCT8/OxR cells were transfected with sh-NC, sh-MALAT1, sh-MALAT1 + anti-miR-NC, or sh-MALAT1 + anti-miR-324-3p. h, i The measurement of IC50 value in HCT116/OxR and HCT8/OxR cells response to Ox. j, k Detection of cell viability by CCK-8 assay. l, m Measurement of cell migration using transwell assay. n Detection of cell apoptosis by flow cytometry. o, p The expression of Bcl-2, Cleaved caspase-3, E-cadherin and Vimentin was determined by western blot. N = 3, *P < 0.05