Fig. 5

Low expression of miR-367 upregulates the expression of LPA1 to promote proliferation, invasion, and angiogenesis of ovarian cancer A2780 cells. a RT-qPCR analysis results of the efficiency of LPA1 silencing by appropriate siRNAs. b miR-367 and LPA1 expression in cells determined by using RT-qPCR. c A2780 cell proliferation ability detected by Transwell assay (scale bar = 25 μm). d Invasion ability of A2780 cells measured by Transwell assay (scale bar = 50 μm). e Relative length of blood vessels. f Relative number of blood vessel branches. g–j Statistics of CAM blood vessel number, area, vessel area ratio and tissues area. k RT-qPCR detection of mRNA levels of related factors. l Western blot analysis of protein levels of related factors. m Statistics of protein expression levels of related factors. Measurement data were expressed as mean ± standard deviation. Data in compliance with normal distribution and homogeneity among multiple groups were conducted by one-way ANOVA followed by Tukey’s post hoc test. *p < 0.05 vs. NC. ^#p < 0.05 vs. cells treated with si-LPA1. The experiment was repeated three times independently