Fig. 2
From: XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress
ER stress-induced decrease in CENPF expression is dependent on ATF6 and XBP1. a q-RT-PCR analysis of CENPF mRNA level in U2OS cells with or without transient knockdown of IRE1α, PERK and ATF6. b Western blotting analysis of CENPF, IRE1α, PERK and ATF6 protein level in U2OS cells with or without transient knockdown of IRE1α, PERK and ATF6. c q-RT-PCR analysis of CENPF mRNA level in U2OS cells with or without transient knockdown CHOP, XBP1 and ATF4. d Western blotting analysis of CENPF protein level in U2OS cells with or without transient knockdown of CHOP, XBP1 and ATF4. e 1 μg XBP1 spliced expression vector was transiently transfected into U2OS cells as indicated, and the mRNA level was quantified by q-RT-PCR analysis. f Different dose of XBP1 spliced expression vector was transiently transfected into U2OS cells as indicated, and CENPF protein level was estimated by western blot analysis. Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001