Fig. 3

Transient transfection analysis of CENPF gene promoter constructs. a Using TRANSFAC-TESS and Match™ on-line softwares, we identified potential transcription factor binding motifs in the CENPF promoter. b Schematic representation of a series of 5′-deletion CENPF promoter luciferase constructs. Numbering is defined relative to the transcription start site. c Construction of a series of 5′-truncated CENPF promoters and their relative luciferase activities without (the white bar) or with (the black bar) 24 h TG treatment. Luciferase activities were determined and normalized to Renilla activity. Results are expressed as a percentage of the untreated control, which is taken as 100%. Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001