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Fig. 4 | Cancer Cell International

Fig. 4

From: XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress

Fig. 4

ChIP and ChIP Re-IP assays analysis of TG-induced binding of XBP1 and ATF6 to the CENPF promoter in vivo. a ChIP assay to examine binding of XBP1 to the CENPF promoter region (− 679 to − 488) by TG in vivo. b ChIP assay to examine binding of XBP1 to the CENPF promoter (− 241 to − 78) by TG in vivo. c ChIP Re-IP to examine whether XBP1and ATF6 were assembled on the same CENPF promoter region (− 679 to − 488). d ChIP Re-IP to examine whether XBP1and ATF6 were assembled on CENPF promoter region (− 241 to − 78). Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001

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