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Fig. 5 | Cancer Cell International

Fig. 5

From: XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress

Fig. 5

Mutagenesis analysis of XBP1 binding motifs involved in TG-induced transcriptional activity of the CENPF promoter. a Construction of CENPF promoter/luc vectors with XBP1 binding elements. Wild-type, mutant and deletion XBP1 binding elements (position − 567) are denoted as p680-luc, p680m-luc and p680d-luc, respectively. Wild-type, mutant and deletion XBP1 binding element (position − 192) are denoted by p242-luc, p242m-luc and p242d-luc, respectively. b, c Relative luciferase activities with or without TG treatment for 24 h. Measured luciferase activities were normalized to Renilla luciferase activities. Results are expressed as a percentage of the untreated control that is taken as 100%. Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001

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