Fig. 6
From: XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress
Overexpression of CENPF inhibits cell apoptosis and promotes cell proliferation in response to ER stress. a U2OS cells transfected with or without transient overexpression of CENPF were incubated with 1 μM TG for 24 h, and apoptotic cells were counted using a TUNEL assay. b U2OS cells transfected with or without transient overexpression of CENPF were incubated with or without TG for 24 h. Viable cells were counted using trypan blue exclusion. c U2OS cells transfected with control or CENPF plasmid were incubated with or without TG for 7 days. Colonies were stained with crystal violet and counted. Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001