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Fig. 1 | Cancer Cell International

Fig. 1

From: Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis

Fig. 1

Circ_0000285 expression was upregulated in OS. a QRT-PCR was used to detect the expression of circ_0000285 in normal tissues and tumor tissues. b QRT-PCR was used to detect the expression of circ_0000285 in normal cells (hFOB 1.19) and tumor cells (SJSA1 and U2OS). c Western blot was used to detect whether PARP and GAPDH protein were in total (protein), Cytoplasm and Nucleus. d qRT-PCR determined that circ_0000285 expressed high in cytoplasm in SJSA1 and U2OS cells. e, f qRT-PCR was used to detect the expression of circ_0000285 with RNase R and Actinomycin D treated RNA in SJSA1 and U2OS cells. *P < 0.05, **P < 0.01

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