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Fig. 3 | Cancer Cell International

Fig. 3

From: Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis

Fig. 3

Circ_0000285 sponged miR-409-3p to target IGFBP3 in OS. a qRT-PCR was used to detect the expression of miR-32-3p, miR-548-5p, miR-127-5p, miR-1278, miR-2467-3p, miR-4731-5p, miR-599 and miR-409-3p in si-NC and si-circ_0000285 groups in SJSA1 and U2OS cells. b The predicted binding sites of miR-409-3p to the circ_0000285 and IGFBP3 sequences were shown. c, d Luciferase activities were detected in SJSA1 and U2OS cells co-transfected miR-409-3p mimic or miRNA NC with WT-circ_0000285 or MUT-circ_0000285 and WT-IGFBP3-3′UTR or MUT-IGFBP3-3′UTR. e, f RIP assay was applied to determine the relationship among circ_0000285, miR-409-3p and IGFBP3. *P < 0.05, **P < 0.01, ***P < 0.001

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