Fig. 4

The expression of miR-409-3p was reduced while the expression of IGFBP3was increased in OS. a The expression of miR-409-3p was detected in normal tissues and tumor tissues with qRT-PCR. b The expression of miR-409-3p was detected in normal cells and tumor cells with qRT-PCR. c The expression of miR-409-3p was measured in inhibitor NC and miR-409-3p inhibitor groups with qRT-PCR. d The expression of miR-409-3p was measured in si-NC, si-circ_0000285, si-circ_0000285 + inhibitor NC and si-circ_0000285 + miR-409-3p inhibitor groups with qRT-PCR. e The expression of IGFBP3 was detected in normal tissues and tumor tissues with western blot. f The expression of IGFBP3 was detected in normal cells and tumor cells with western blot. g The expression of IGFBP3 was detected in si-NC and si-IGFBP3 groups with western blot. h The expression of IGFBP3 was detected in inhibitor NC, miR-409-3p inhibitor, miR-409-3p inhibitor + si-NC and miR-409-3p inhibitor + si-IGFBP3 groups with western blot. i The expression of IGFBP3 was detected in si-NC, si-circ_0000285, si-circ_0000285 + pc-NC and si-circ_0000285 + pc-IGFBP3 groups with western blot. j The expression of IGFBP3 was detected in si-NC, si-circ_0000285, si-circ_0000285 + inhibitor NC and si-circ_0000285 + miR-409-3p inhibitor groups with western blot. *P < 0.05