Table 1 The primers used in the quantitative real-time PCR assay
From: The novel circ_0028171/miR-218-5p/IKBKB axis promotes osteosarcoma cancer progression
Name | Sequence (5′ →  3′) |
|---|---|
circRNA_0028171 | |
 Forward | CAGGGCAGGGACAGGAAG |
 Reverse | GAGACAGGCAAGAGGACAAGG |
GAPDH | |
 Forward | TGTGGGCATCAATGGATTTGG |
 Reverse | ACACCATGTATTCCGGGTCAAT |
IKBKB | |
 Forward | GGAAGTACCTGAACCAGTTTGAG |
 Reverse | GCAGGACGATGTTTTCTGGCT |
U6 | |
 Forward | CTCGCTTCGGCAGCACA |
 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGGAAC |
miR-218-5p | |
 Forward | GGGGGTTGTGCTTGATCTAAC |
 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACACATGGT |
miR-1273e | |
 Forward | ACACTCCAGCTGGGTTGCTTGAACCCAGGA |
 RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCCACTTC |
miR-583-5p | |
 Forward | GGTGCTCTATGGTAATCTAGCTG |
 RT | GTTCAGCCTAGTGCAGGGTCAGAGGTGCAGAGGAGACGACTTATACA |
miR-3919 | |
 Forward | GGTAGAGTTCTCTCAGTAGTAAC |
 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAGGGATT |
miR-6835-5p | |
 Forward | AGTCGAAGTCGAAGCTGTCATCG |
 RT | GGTCAGGTCAACTTGCACCTGCAAACTGTGGTAGGCTTAACCGTCATC |
miR-6748-5p | |
 Forward | ATCGGATCAATGCTTAGCTAGAG |
 RT | GGCCTAAATGTAACATGCTAGCTAGTTGGATAAATGGTCCGATCGATC |