Fig. 6

Reversal of M-CMs by downregulation of let-7b-5p inhibits the proliferation of PCa cells. M1 and M-CMs were transfected with let-7b-5p mimics or inhibitors, respectively, for 72 h. Then CM from macrophages was collected and added to the PC-3 cells. a PC-3 cells were exposed to CM from macrophages of different groups or RPMI 1640 medium as a control for 48 or 72 h. We measured viability of the PC-3 cells with the CCK-8 assay, and counted the number of viable cells relative to the control group. *P < 0.05; **P < 0.01. b After 2 weeks of incubation, CM from M-CMs treated with let-7b-5p inhibitors significantly decreased PC-3 colony number compared with the conditioned medium from M-CM. Each value represents the mean ± SEM. *P < 0.05, **P < 0.01