Fig. 2

LOXL1-AS1 sponges miR-589-5p in laryngocarcinoma cells. a, b Nuclear separation and FISH experiments confirmed the main cytoplasmic location of LOXL1-AS1 in both Tu-177 and M4E cells. c RNA pull down assay detected that the high enrichment of miR-589-5p but not miR-423-5p in Bio-LOXL1-AS1 groups. d Ago2-RIP experiments illustrated the strong enrichment of LOXL1-AS1 and miR-589-5p in anti-Ago2 groups. e A binding site of miR-589-5p to LOXL1-AS1 was presented by using starBase. f Luciferase reporter assays examined the declined luciferase activity of pmirGLO/LOXL1-AS1-WT but not pmirGLO or pmirGLO/LOXL1-AS1-Mut under the overexpression of miR-589-5p. The standard deviation of control groups in c, d and f was calculated by 2^−ΔΔCt method as follow: ΔCt (Control) = ΔCt (target, Control) – ΔCt (reference, Control); mean ΔCt (Control) = {ΔCt−1 (Control) + ΔCt−2 (Control) + ΔCt−3 (Control)}/3; ΔΔCt (Control) = ΔCt (Control)−mean ΔCt (Control). **p < 0.01