Fig. 3

MiR-589-5p restrains cell proliferation, migration and EMT in laryngocarcinoma. a, b CCK8 and colony formation assays examined the repressive impact of overexpressed miR-589-5p on the proliferation of Tu-177 and M4E cells. c, d. Wound healing and transwell assays manifested the hampered capacity of migration in Tu-177 and M4E cells in face of up-regulation of miR-589-5p. Relative wound width in c was calculated using the relative value of wound widths at 24 h to that at 0 h. e IF assessed the augmented expression of E-cadherin and declined level of N-cadherin in Tu-177 and M4E cells with up-regulated miR-589-5p. The standard deviation of control groups in Figure was calculated by 2^−ΔΔCt method as follow: ΔCt (Control) = ΔCt (target, Control) – ΔCt (reference, Control); mean ΔCt (Control) = {ΔCt−1 (Control) + ΔCt−2 (Control) + ΔCt−3 (Control)}/3; ΔΔCt (Control) = ΔCt (Control)−mean ΔCt (Control). **p < 0.01