Fig. 2

Glioma cells-derived exosomal miR-148a-3p promotes HUVEC proliferation and tube formation. a miR-148a-3p expression was determined by RT-qPCR in the exosomes isolated from U251 cells or exosomes from U251 cells transfected with miR-148a-3p inhibitor (U6 was used as internal control). b Uptake of PKH67-labeled U251-derived exosomes by HUVECs visualized under LSCM (scale bar = 25 µm). c miR-148a-3p expression was determined by RT-qPCR in the HUVECs after co-culture with exosomes from U251 cells transfected with miR-148a-3p inhibitor (U6 was used as internal control). d CCK-8 detection of HUVEC proliferation in the co-culture system of HUVECs and exosomes from U251 cells transfected with miR-148a-3p inhibitor. e Lumen formation ability of HUVECs in the co-culture system of HUVECs and exosomes from U251 cells transfected with miR-148a-3p inhibitor (scale bar = 100 µm). f ELISA detection of VEGF and VEGFR2 expression in cell supernatant in the co-culture system of HUVECs and exosomes from U251 cells transfected with miR-148a-3p inhibitor. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. U251 mentioned in this figure refers to U251-MG. Data are shown as mean ± SEM. The cell experiments were performed in 3 repeats and each repeat was performed in technical replicate (triplicate). The unpaired t test was used for comparison between two groups. The repeated measures ANOVA was used for data analysis at different time points. Comparisons among multiple groups were conducted using one-way ANOVA with Tukey's test