Fig. 3

HUVEC proliferation and angiogenesis could be enhanced by miR-148a-3p. a Expression of miR-148a-3p was determined by RT-qPCR in HUVECs transiently transfected with miR-148a-3p mimic or inhibitor (U6 was used as internal control). b CCK-8 assay to detect proliferation of HUVECs transiently transfected with miR-148a-3p mimic or inhibitor. c Tube formation assay to test the angiogenesis ability of HUVECs transiently transfected with miR-148a-3p mimic or inhibitor (scale bar = 100 µm). d Expression of VEGF and VEGFR2 was tested using ELISA in cell supernatant upon transient transfection with miR-148a-3p mimic or inhibitor. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. the cells transfected with mimic NC or with inhibitor NC. Data are shown as mean ± SEM. The cell experiments were performed in 3 repeats and each repeat was performed in technical replicate (triplicate). Repeated measures ANOVA was used for data analysis among multiple groups and multiple time points. Unpaired t test was used for comparison between the two groups