Fig. 3
From: SOX4 promotes the growth and metastasis of breast cancer
SOX4 binds to the promoter of the CXCR7 gene and activates its transcription. a Increased amount of CXCR7 mRNA in SOX4-expressing MDA-MB-231 and SUM149 cells. The data are presented as the mean ± s.d. of triple wells. Unpaired two-tailed t-test: *p < 0.05, **p < 0.01. b CXCR7 gene fragments amplified from pull-down samples of the chromatin immunoprecipitation assay using the SOX4 antibody and non-specific IgG, and the input samples. c Dual-luciferase reporter assay showing increased CXCR7 promoter-specific reporter activity associated with SOX4 overexpression. d and e CXCR7 inhibitor CCX771 (ccx) reversed the enhancing effect of SOX4 on migration and invasion. The data are presented as the mean ± s.d. of triplicate wells. Unpaired two-tailed t-test: *p < 0.05, ***p < 0.001. f IHC analysis of SOX4 and CXCR7 expression in human breast primary tumors (Primary) and lymph node metastatic foci (LN)