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Fig. 6 | Cancer Cell International

Fig. 6

From: Aging-related tumor associated fibroblasts changes could worsen the prognosis of GBM patients

Fig. 6

Differentially expressed genes (DEGs) showed epithelial mesenchymal transition (EMT) as potential mechanism for prognosis differences between two age group. a Hallmark of enriched pathways related to cancer development were evaluated. Epithelial-mesenchymal transition (EMT) is the most enriched pathway among all. b Gene-set enrichment analysis (GSEA) analysis also showed significant enrichment among patients under and equal and greater than 40 years old. c Volcano plot showed total number of 29 DEGs overlapped between GSEA and DEGs. 14 genes related to EMT were showed in darker color. d LASSO regression further screen prognosis-related genes from the DEGs. Tuning parameter (lambda) screening in the LASSO regression model. The partial likelihood deviance was generated versus log(lambda), and the lowest partial likelihood deviance corresponded to the optimal number of genes. The dotted vertical lines corresponded to the optimal lambda value according to the 1 standard error criteria and the minimum criteria. When 10 genes remained, the partial likelihood deviance was the lowest. e The LASSO coefficient profiles of the 29 genes. A vertical line was drawn at the value chosen by tenfold cross-validation, which indicated that 10 non-zero coefficients were identified by the optimal lambda value. f The expression level of the 7 genes screened out by Lasso regression analysis was significantly higher in patients equal and greater than 40 years old (≥ 40 y, red color) than those in patients under 40 years old (< 40 y, blue color). Wilcxon rank sum test was used as statistical method. **, P < 0.01; g Differentially expressed genes among patients over (≥ 40 y, showed in red) and under 40 years old (< 40 y, showed in blue)

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