Fig. 6

NFIB activated Snail1. a NFIB mRNA and Snail1 mRNA expression had positive correlation in 100 colorectal cancer tissues (r = 0.31, p < 0.001). b In GEPIA database, NFIB mRNA and Snail1 mRNA expression were positively correlated (r = 0.646, p < 0.001). c Expression of Snail1 and NFIB after transfected with NFIB siRNA. d After transfected with NFIB siRNA, the relative protein level of Snail1 were decreased (p < 0.01). e After transfected with NFIB siRNA, the relative mRNA level of Snail1 were decreased (p < 0.01). f CHIP experiment verified that transcription factor NFIB could bind to the promoter region of Snail1 gene. g–i. According to the binding sites predicted by the database, wild-type and mutant plasmids of the Snail1 promoter region were constructed. Double luciferase assay experiment verified that NFIB binded to the wild-type promoter region of Snail1, instead of the mutant type. j–k Expression of Snail1 was detected after co-transfected NFIB siRNA into stable cell lines. The difference was statistically significant between NFIB siRNA-LV inhibitor + LV INC− group and NFIB siRNA-LV inhibitor-LV INC + group (p < 0.001) The difference was not statistically significant between NFIB siRNA + LV inhibitor-LV INC + group and NFIB siRNA + LV inhibitor + LV INC− group (p > 0.05)