Fig. 4

DAAM1 is a target gene of miR-34a-5p. a RT-qPCR evaluated the expression of 3 mRNAs when miR-34a-5p was up-regulated. b DAAM1 mRNA expression was detected in PCa cells and normal RWPE-1 cells by RT-qPCR. c Protein levels of DAAM1 were determined by western blot. d RIP assay was performed to validate that LINC01006, miR-34a-5p and DAAM1 were coexisted in Ago2 pallets. e Bioinformatics tools predicted the binding sites between miR-34a-5p and DAAM1. f, g Interaction between miR-34a-5p and DAAM1 at above binding sites was certified by RNA pull down and luciferase reporter assays. h RT-qPCR measured miR-34a-5p expression when cells were transfected with miR-36a-5p inhibitor. i DAAM1 mRNA expression was determined by RT-qPCR in sh-NC, sh-LINC01006#1 and sh-LINC01006#1 + miR-34a-5p mimics groups. j DAAM1 protein levels were also detected by western blot in sh-NC, sh-LINC01006#1 and sh-LINC01006#1 + miR-34a-5p mimics groups. *P < 0.05; **P < 0.01