Fig. 6

TLR4 binds to YAP in the nucleus by promoting IRF3 phosphorylation, which in turn activates CDX2. a Correlation diagram of TLR4 and IRF3 expression (r = − 0.144, p = 0.00516); b Correlation diagram of IRF3 and YAP (YAP1) expression (r = − 0.138, p = 0.00742); c Western blot analysis of TLR4 and phosphorylation of IRF3 in TNF-α-induced GES-1 cells transfected with sh-or oe-TLR4; d Western blot analysis of phosphorylation of IRF3 in TNF-α-induced GES-1 cells transfected with sh- or oe-TLR4; e Western blot analysis of phosphorylation of IRF3 in TNF-α-induced GES-1 cells transfected with sh- or oe-TLR4; f IRF3 binding to YAP in the nucleus of TNF-α-induced GES-1 cells detected by Co-IP assay; g IRF3 binding to YAP in cells transfected with sh- or oe-TLR4; h Quantitative analysis of CDX2 expression in starBase database, red box on the left indicates the expression in GC samples, purple box indicates the expression in normal samples; i MEM analysis (https://biit.cs.ut.ee/mem/index.cgi) of the relationship between YAP (YAP1) and CDX2 (p < 0.05); j Detection of YAP and CDX2 expression in TNF-α-induced GES-1 cells after YAP silencing; k Western blot analysis of CDX2 protein in TNF-α-induced GES-1 cells after over-expression of TLR4 or silencing of TLR4; *p < 0.05, vs. cells transfected with oe-NC or sh-NC. Measurement data (mean ± standard deviation) between two groups were compared with unpaired t test while data between multiple groups were compared with one-way ANOVA. Cell experiments were repeated three times