Fig. 2
From: Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity
The invasion, migration and proliferation of RM-1 cells. a The cells that penetrated the matrix-coated membrane. The ability of invasion was examined by transwell assay. b Mean cell count (per field) that penetrated the matrix-coated membrane. The results are reported as the mean of triplicate assays. IL-35 promoted invasion of RM-1 cells and IL-35 neutralizing antibody decreased RM-1 invasion. c The migration ability of RM-1 cells was detected using the wound healing assay. Cell migration was calculated as the area of cell migration compared to the area of the initial wound. The results are reported as the mean of triplicate assays. d The proliferation of RM-1 cells were examined by CCK-8 assay. The experiment was independently performed three times. ***p < 0.001