Table 1 Experimental verification of peptides
From: Emerging role of long noncoding RNA-encoded micropeptides in cancer
Techniques | Advantages | Disadvantages |
|---|---|---|
Ribo-seq | Discover more potential peptides | Indirect evidence of translation, expensive, poor repeatability |
RNC-seq | High sequence coverage and detection efficiency | Low sequencing accuracy, unable to obtain ribosome and ORF information |
Mass spectrometry | Strong evidence, detect translation directly | Require specific expertise, rely on protein databases to search |
Fusion protein | Easy to detect | May affect the structure and function of original protein |