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Fig. 1 | Cancer Cell International

Fig. 1

From: DARS-AS1 accelerates the proliferation of cervical cancer cells via miR-628-5p/JAG1 axis to activate Notch pathway

Fig. 1

DARS-AS1 promotes cell proliferation and restrains cell apoptosis in CC. a GEPIA data indicated that DARS-AS1 level was enhanced in 306 CC tissues relative to 13 normal cervical tissues. b RT-qPCR proved that the expression of DARS-AS1 was elevated in CC cell lines (HeLa, C33A, MS751, SiHa ME-180 and CaSki) compared to human normal cervical epithelial cell line (Ect1/E6E7). c RT-qPCR evaluated the declined DARS-AS1 expression in HeLa and CaSki cells transfected with sh-DARS-AS1#1 or sh-DARS-AS1#2. d CCK-8 assay confirmed the suppressive effects of DARS-AS1 depletion on the viability of HeLa and CaSki cells. e Colony formation assay proved the proliferation capacities of HeLa and CaSki cells was impaired in the case of DARS-AS1 knockdown. f TUNEL assay detected that the apoptosis rate of HeLa and CaSki cells was heightened with DARS-AS1 inhibition. Scale bar = 100 μm. g Flow cytometry analysis assessed the promoted apoptosis ability of HeLa and CaSki cells in the case of DARS-AS1 knockdown. Data from three independent experiments were exhibited mean ± SD. *P < 0.05, **P < 0.01

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