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Fig. 3 | Cancer Cell International

Fig. 3

From: DARS-AS1 accelerates the proliferation of cervical cancer cells via miR-628-5p/JAG1 axis to activate Notch pathway

Fig. 3

DARS-AS1 modulates JAG1 to activate Notch pathway by binding with miR-628-5p. a ENCORI predicted 5 potential target genes of miR-628-5p. b RT-qPCR unveiled that only the expression levels of JAG1 was markedly reduced in CC cells transfected with miR-628-5p mimics. c The binding sites between miR-628-5p-WT and JAG1-WT were exhibited by ENCORI, and the sequences of miR-628-5p-Mut and JAG1-Mut were designed. d RBP-JK luciferase reporter assay and western blot analysis confirmed the inactivation of DARS-AS1 depletion on Notch pathway. e RIP assay followed by RT-qPCR confirmed the strong enrichment of DARS-AS1, miR-628-5p and JAG1 in Ago2-involved RISC in CC cells. f RNA pull down assay disclosed the high enrichment of DARS-AS1 and JAG1 in Bio-miR-628-5p-WT groups in CC cells. g The combination between JAG1 and miR-628-5p at predicted binding sites was verified via luciferase reporter assay. h Luciferase reporter assay determined that DARS-AS1 competed with JAG1 to interact with miR-628-5p in CC cells. All data were displayed as the mean ± SD from three independent experiments. **P < 0.01, n.s.: no significance

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