Fig. 4

TRAF3 is a direct target of miR-1307-5p in LUAD cells. a The binding degree of miR-1307-5p to TRAF3 was detected by double luciferase assay. MiR-1307-5p targets TRAF3. Compared with the control group, *P < 0.05 and **P < 0.01. b, c Western blot was used to detect the difference of TRAF3 protein levels in the treatment groups after transient transfection of H1299 and A549 lung adenocarcinoma cells. Overexpression of miR-1307-5p downregulated the protein expression level of TRAF3 (b) and inhibited the upregulated protein expression level of TRAF3 (c). Compared with the control group, *P < 0.05 and **P < 0.01. d, e QPCR was used to detect the difference of TRAF3 mRNA level between the treatment groups after transient transfection of H1299 and A549 lung adenocarcinoma cells. Overexpression of miR-1307-5p downregulated the mRNA expression level of TRAF3 (d) and inhibited the upregulated mRNA expression level of TRAF3 (e) by miR-1307-5p. Compared with the control group, *P < 0.05, **P < 0.01 and ***P < 0.005. Data are expressed as mean ± standard deviation. The experiment was repeated three times