Fig. 5

SLC2A3 promotes GC progression by inducing macrophage infiltration and M2 subtype transition. a The relationship between SLC2A3 expression and infiltrating levels of B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells. b Scatterplots of the correlations between SLC2A3 expression and macrophage M1 and M2 subtype gene markers obtained using the TIMER database. c RT-PCR assays revealed M1 and M2 macrophage subtype marker expression in THP1 cells after co-culture with SLC2A3 overexpressing and knockdown cells. d Transwell analysis. e THP-1 cells were incubated with supernatant from cells transfected with SLC2A3 overexpression plasmid or siRNA. Cells were then fixed and immunolabeled for CD68 or CD163. Nuclei were stained with DAPI (blue). f RT-PCR assay revealed M1 and M2 macrophage subtype marker expression in THP1 cells treated with or without 10 mmol/ml lactate for 24 h. Statistical results are shown as mean ± SD, *ρ < .05, **ρ < .001, ****ρ < .001, based on two-way ANOVA or Student’s t-test