Fig. 2

NEO1 was down-regulated in CRC patients. a Quantitative real-time PCR analysis showed that NEO1 was down-regulated in 53 CRC tissues compared to paired adjacent tissues (p = 0.0038). b Western blotting analysis demonstrated that NEO1 was down-regulated in 4 CRC tissues. The gray intensity of protein expression was quantified by image J software. NEO1 protein expression value was calculated against GAPDH. c-f The expression of NEO1 in CRC was negatively associated with the tumor stage in TCGA (c, d), GSE41258 datasets (e) and Zhongnan Hospital tissues (f). **P < 0.01