Fig. 4

Transfection efficiency of NEO1 plasmids and siRNAs in CRC cells. a The mRNA and protein expression levels of NEO1 in three CRC cell lines were tested by real-time PCR and western blotting. b Reverse transcription PCR and western blotting analysis demonstrated that NEO1 was significantly over-expressed in DLD1 and HCT116 cells transfected with NEO1 plasmids (NEO1 OE) compared with empty plasmids (Vec). c Reverse transcription PCR and western blotting analysis demonstrated that NEO1 expression was reduced in SW480 cells transfected with NEO1 siRNAs (siNEO1 #1, #2) compared with negative control siRNAs (siNC). Data shown above are presented as the means ± s.e.m., n = 3; *P < 0.05, ***P < 0.001