Fig. 1

SNHG4 expression in human RCC tissue samples, and its association with prognosis. a qRT-PCR assay analysis of SNHG4 expression in TT and ANT human renal tissues in our cohort (n = 99). b–g qRT-PCR assay showing that high expression of SNHG4 was significantly associated with aggressive T stage (b), node invasion (c), metastasis status (d), poor tumor grade (e), UISS score category (f), and relapse status (g). Data were obtained using the 2^−ΔΔCT method and were normalized to GAPDH levels. h–j Kaplan–Meier survival curves according to high and low SNHG4 expression in patients with RCC from the Zhengzhou cohort (h, i) and TCGA-KIRC data set (j). Tumor classification and stage were referred to the 7th edition of UICC (2009) on cancer staging system. RCC: renal cell carcinoma; TT: tumor tissues; ANT: adjacent non-cancerous tissues; UISS: University of California, Los Angeles, Integrated Staging System; TCGA: the cancer genome atlas; KIRC: kidney renal clear cell carcinoma. *P < 0.05; **P < 0.01; ***P < 0.001