Fig. 3
Knockdown of SNHG4 inhibits cell proliferation, migration, invasion, but promotes cell apoptosis in RCC cell lines. a qRT-PCR assay analysis of the level of SNHG4 in 769-P and ACHN cell lines after transfection with two parallel siSNHG4 (siSNHG4#1 and siSNHG4#2) or siSCR. b, c CCK-8 assay (b) and Western blot assay (c) analysis of the proliferative ability of 769-P and ACHN cells transfected with siSNHG4#1 or siSCR. d The activity of caspase-3, -8, and -9 assay analysis of cell apoptosis of RCC cell lines after transfection with the indicated vectors. e The wound healing assay analysis of cell migration in 769-P and ACHN cell lines after transfection with the indicated vectors. f, g The transwell assay without (f) or with (g) matrigel analysis of cell migration and invasion in 769-P and ACHN cell lines after transfection with the indicated vectors. Data are presented as mean ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. SCR: scramble; RCC: renal cell carcinoma. *P < 0.05; **P < 0.01; ***P < 0.001