Fig. 4

SNHG4 acts as a sponge of miR-204-5p in RCC cell lines. a Predicted subcellular localization of SNHG4 in RCC cells using the “lnclocator” algorithm. b qRT-PCR assay analysis of the subcellular localization of SNHG4 in RCC cells. GAPDH and U1 served as a cytoplasmic and nuclear localization marker, respectively. c The predicted binding sites of miR-204-5p with SNHG4 wt or mut were showed. d Luciferase activity assay analysis of 769-P and ACHN cell lines co-transfected with miR-204-5p mimic or inhibitor with SNHG4 wt or mut reporter gene. e Anti-Ago2 RIP assay analysis of 769-P and ACHN cell lines with transiently miR-204-5p mimic and control. The expression levels of endogenous SNHG4 were detected using qRT-PCR. f qRT‐PCR assay analysis of the expression of miR-204-5p in 769-P and ACHN cell lines after transfection with the indicated vectors. g Spearman’s correlation analysis of the correlation between SNHG4 and miR-204-5p in RCC tissues from our cohort. Data are presented as mean ± standard deviation from triplicate experiments. A t-test was used to evaluate the statistical significance as compared to the control. SCR: scramble; RCC: renal cell carcinoma; ns, not significant. *P < 0.05; **P < 0.01; ***P < 0.001